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8?Effects of different administration routes on the action of magnesium sulfate 224Section 9?Determination of IC50 of drugs in vitro 228Chapter 2¡¡Pharmacology of efferent nervous system 234Section 1?Dose-effect relationship of acetylcholine 234Section 2?The effect of efferent nervous system drugs on isolated intestinal smooth muscle 238Section 3?The effect of efferent nervous system drugs on the pupil of rabbits 243Section 4?Inhibition of atropine on smooth muscle in mice 247Section 5?Poisoning and rescue of organophosphorus pesticides 253Chapter 3¡¡Central nervous system pharmacology 258Section 1?Analgesia experiment of drugs 258Section 2?The effect of chlorpromazine on electric stimulation-induced agitation in mice 263Section 3?Effects of aspirin and chlorpromazine on body temperature 266Section 4?Comparison of the effects of pancuronium bromide and succinylcholine on muscle relaxation in rabbits 272Section 5?Anticonvulsant effect of phenobarbital sodium in mice 276Section 6?The effect of drugs on resisting nikethamide-induced convulsions 282Section 7?Anxiolytic effects of diazepam 287Section 8?Rescue effect of nikethamide on morphine induced respiratory depression 292Section 9?Local and systemic effects of procaine 296Chapter 4¡¡Cardiovascular system pharmacology 301Section 1?Effect of efferent nervous system drugs on blood pressure in rabbits 301Section 2?The effect of lidocaine on arrhythmia induced by electrical stimulation 307Section 3?The therapeutic effect of antiarrhythmic drugs on BaCl2-induced arrhythmia 310Section 4?The effect of cardiac glycosides on the relaxation and contraction of detached frog hearts 314Section 5?Effects of propranolol on hypoxia tolerance in mice 319Chapter 5¡¡Hormonal visceral pharmacology 324Section 1?Antagonistic effect of protamine sulfate on heparin anticoagulant activity 324Section 2?Reaction and rescue of insulin overdose 328Section 3?The antagonistic effect of aspirin on thrombosis formation 332Section 4?The effect of oxytocin on uterine smooth muscle in vitro in guinea pigs 337Section 5?The effect of diuretics on animal urine output 341Section 6?The effect of hydrocortisone on ear swelling induced by paraxylene in mice 345Section 7?Effects of magnesium sulfate and raw rhubarb on intestinal propulsion in mice 350Section 8?The effect of drugs on coagulation time in mice 354Section 9?Observation of antitussive effect of pentoverine 359Chapter 6¡¡Pharmacology of chemotherapy drugs 364Section 1?Toxic reactions of streptomycin and its relief 364Section 2?Determination of blood concentration of cefradine 367Section 3?Hemolysis test of injection 371Section 4?Pyrogen examination of injection 376Section 5?The inhibitory effect of doxorubicin on tumor cell growth in vitro 381Section 6?The in vitro antibacterial effect of penicillin G 385Chapter 7¡¡Experimental design 392Section 1?The objective and significance of experimental design 392Section 2?The basic elements and principles of experimental design 393Section 3?The main content and method of experimental design 398Section 4?Types of experimental design 400Section 5?The procedure and requirement of experimental design 402Chapter 8¡¡Case analysis 405Case 1 405Case 2 406Case 3 407Case 4 407Chapter 9¡¡Common experimental instruments 409Section 1?Information integrated signal acquisition and processing system 409Section 2?Spectrophotometer 414Section 3?Physiological and pharmacological electronic stimulators 417Appendix 422Appendix 1?Conversion of drug concentration and dosage in pharmacological experiments 422Appendix 2?Composition and preparation of common physiological solutions 425Appendix 3?Drug dosage form and prescription 426Appendix 4?Experimental report writing 430