Modernanimalreproductivetechnology

Introduction Chapter 1 Estrus and Ovulation Control 1.1 Principle of estrus diagnosis 1.1.1 General diagnosis technology 1.1.2 Modern estrus monitoring technology 1.2 Estrus control technology 1.2.1 Estrus induction technology 1.2.2 Simultaneous estrus technology 1.3 Ovulation control technology 1.3.1 Ovulation timing control and Ovsynch 1.3.2 Ovulation count control Chapter 2 Fertilization Technology 2.1 Artificial insemination 2.1.1 Advantages and disadvantages of artificial insemination 2.1.2 Brief introduction of development history for artificial insemination technology in livestock and poultry 2.1.3 Technical process of artificial insemination 2.2 In vitro fertilization technique 2.2.1 A brief history of in vitro fertilization 2.2.2 In vitro maturation of oocytes 2.2.3 In vitro capacitation of sperm 2.2.4 Procedure of in vitro fertilization 2.2.5 Main factors affecting in vitro fertilization 2.2.6 Application, problems, and prospects of in vitro fertilization 2.3 Microinsemination 2.3.1 Operating procedure 2.3.2 Significance of MI Chapter 3 Pregnancy Diagnosis 3.1 Basis for pregnancy diagnosis 3.2 Principles of pregnancy diagnosis 3.3 Methods of pregnancy diagnosis 3.3.1 Routine techniques for pregnancy diagnosis 3.3.2 Smart pregnancy diagnosis techniques Chapter 4 Parturition Control 4.1 Principle of parturition control 4.2 Application of parturition control 4.3 Significance of parturition control 4.4 Methods of parturition control 4.4.1 Treatment scheme of prostaglandin 4.4.2 Treatment scheme of oxytocin 4.4.3 Treatment scheme of eorticosteroid Chapter 5 Oocyte and Embryo Cryopreservation 5.1 History for embryo and oocyte cryopreservation 5.1.1 Oocyte cryopreservation 5.1.2 Embryo cryopreservation 5.2 Principle and technology of cryopreservation for ova and embryos 5.2.1 Technical terms 5.2.2 Changes during freezing and cryoinjuries 5.2.3 Cryoprotectants 5.2.4 Principles of cryopreservation 5.3 Procedures for embryo cryopreservation 5.3.1 Conventional freezing 5.3.2 Ultra-rapid freezing 5.3.3 Vitrification cryopreservation procedure 5.4 Procedures for oocyte cryopreservation 5.4.1 Slow freezing 5.4.2 Vitrification cryopreservation 5.5 Prospects and conclusions of oocyte cryopreservation Chapter 6 Sex Control in Mammals 6.1 Brief history of studies on sex determination 6.2 Sex control techniques in mammals 6.2.1 Sex control before fertilization--the separation of X sperm and Y sperm 6.2.2 Internal female sexual manipulation of the female reproductive tract 6.2.3 Mating with semen by RNAi injection into testis 6.2.4 Sex identification of pre-implanted embryo 6.2.5 Sex control by ultrasonography identification 6.2.6 Other biotechniques 6.3 Application prospect of sex control techniques Chapter 7 Embryo Transfer 7.1 Research history of embryo transfer 7.1.1 Development of fresh embryo and embryo transfer 7.1.2 Development of frozen embryo transfer 7.1.3 Development of combination of micromanipulation and embryo transfer 7.1.4 Embryo transfer organizations contributed to the technique development 7.2 Rationale of embryo transfer 7.2.1 Gestational development of reproductive organs after estrus 7.2.2 Pre-implantation status of an early embryo 7.2.3 Immune tolerance of the maternal uterus to the embryo 7.2.4 Pregnancy signals are emitted by the embryo 7.2.5 Stability of embryonic genetic material 7.3 Principles of embryo transfer 7.3.1 Physiological basis of embryo transfer 7.3.2 Time frame for embryo transfer 7.3.3 Strict quality control of the embryo transfer process 7.4 Factors affecting the success of embryo transfer 7.4.1 Maternal hormonal environment required for embryo implantation 7.4.2 Embryonic preparation for implantation necessitates "blastocyst activation" 7.4.3 Uterine receptivity 7.4.4 Cell-cell interactions 7.5 Procedure of embryo transfer in farm animals 7.5.1 Selection of donor 7.5.2 Selection of recipient 7.5.3 Superovulation of donor 7.5.4 Artificial insemination of donor 7.5.5 Synchronization of recipient 7.5.6 Recovery of embryos 7.5.7 Embryo quality control 7.5.8 Embryo transfer to recipient 7.5.9 Post-operative care of donor and recipient 7.6 Main problems of embryo transfer 7.6.1 Less consistent super-rowing results 7.6.2 Low embryo recovery rate 7.6.3 Donor reuse problems 7.6.4 Supporting technology problems 7.6.5 Lack of technical personnel and inadequate promotion system Chapter 8 Stem Cell 8.1 Research history of stem cells 8.2 Morphological and functional characteristics of stem cells 8.2.1 Morphological and functional characteristics of mesenchymal stem cells 8.2.2 Morphological and functional characteristics of embryonic stem cells ~ 8.2.3 Morphological and functional characteristics of iPS cells 8.3 Technical points of stem cell isolation and culture 8.3.1 Main points of culture techniques for mesenchymal stem cells 8.3.2 Main points of culture techniques for embryonic stem cells 8.3.3 Technical points of iPS cell culture 8.4 Main problems of stem cell technology 8.4.1 Main problems of mesenchymal stem cell technology 8.4.2 Main problems of ES cell technology 8.4.3 Main problems existing in iPS cell technology 8.5 Application prospects of stem cell technology 8.5.1 Application prospects of mesenchymal stem cells technology 8.5.2 Application prospects of embryonic stem cell technology 8.5.3 Application prospects of iPS cell technology Chapter 9 Animal Cloning 9.1 Parthenogenesis 9.1.1 PA of ZP-free oocytes in pigs 9.1.2 PA of ZP-intact oocytes in pigs 9.2 Embryos splitting 9.2.1 Basic procedure to split embryos 9.2.2 Significance and application of embryos splitting 9.3 Nuclear transfer 9.3.1 Brief introduction of nuclear transfer 9.3.2 Somatic cell nuclear transfer 9.4 Main problems of animal cloning 9.5 Significance and application of animal cloning Chapter 10 Transgenic Animal 10.1 Point and application of transgenic animal technology 10.1.1 Application of transgenic animal technology in livestock 10.1.2 Application of transgenic animal technology in biology 10.1.3 Application of transgenic animal technology in pharmaceuticals 10.2 Main technical links of transgenic animal technology 10.2.1 Targeting gene selection 10.2.2 Gene modification and expression vector construction 10.2.3 Introduction of exogenous genes 10.2.4 Gene integration and expression analysis 10.2.5 New progress in the research of animal transgenic technology 10.3 Main questions and developmental trend in transgenic animal technology 10.3.1 Main question in transgenic animal technology 10.3.2 Developmental trend in transgenic animal technology Chapter 11 Hormone Immunization and Genetic Immunization 11.1 Hormone immunization 11.1.1 Steroid hormone immunization 11.1.2 Inhibin immunization 11.1.3 Gonadotropin inhibiting hormone immunization 11.1.4 Anti-Mtillerian hormone immunization 11.1.5 Immunocastration 11.2 Genetic immunization 11.2.1 Principle of genetic immunization 11.2.2 Methods of genetic immunization ~ 11.2.3 Advantages and safety of genetic immunization 11.2.4 Genetic immunization for regulating animal reproduction Chapter 12 Avian Reproductive Biotechnology 12.1 Techniques to control avian seasonal reproduction 12.1.1 Mechanism of avian reproductive seasonality 12.1.2 Procedures to control avian seasonal reproduction 12.2 Avian embryo manipulation technique 12.2.1 Artificial insemination and embryo transplantation 12.2.2 Culture of avian embryos in the early stage 12.3 Avian chimeric technology 12.3.1 Classification of avian chimeras 12.3.2 Production of avian chimeras 12.3.3 Identification of chimeras in poultry 12.4 Sex control technology of poultry 12.4.1 Sex reversal 12.4.2 Sex identification 12.4.3 Problems of sex control 12.5 Transgenic technology of avian 12.5.1 Method of gamete vector 12.5.2 Technique of microinjection for fertilized egg 12.5.3 Transfection of embryonic placental cells using retroviral vectors 12.5.4 Transfection of blastoderm cells with lipofectin packaging vectors 12.5.5 Method of blastoderm cell and primordial germ cell vectors 12.5.6 Problems and prospects Abbreviations References