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Ä¿ ¼ÉÏ ¾í**²¿·Ö ÓãÀà»ùÒòתÒÆÄ£Ðͽ¨Á¢Novel Gene Transfer into the Fertilized Eggs of Goldfish (Carassius auratus L 1758) 3ÈËÉú³¤¼¤ËØ»ùÒòÔÚÄàöúÊܾ«ÂÑÏÔ΢עÉäתÒƺóµÄÉúÎïѧЧӦ 8Introduction of Novel Genes into Fish 11ת»ùÒòÓãÄ£Ð͵Ľ¨Á¢ 23µÚ¶þ²¿·Ö ÍâÔ´»ùÒòµÄÕûºÏÓë±í´ïThe ¦Â-Actin Gene of Carp (Ctenopharyngodon idella) 35Isolation and Characterization of ¦Â-Actin Gene of Carp (Cyprinus carpio) 37Primary-Structural and Evolutionary Analyses of the Growth-Hormone Gene from GrassCarp (Ctenopharyngodon idellus) 54Time Course of Foreign Gene Integration and Expression in Transgenic Fish Embryos 64Transgenes in F4 pMThGH-Transgenic Common Carp (Cyprinus carpio L ) are HighlyPolymorphic 73Sequences of Transgene Insertion Sites in Transgenic F4 Common Carp 84Characterization of Transgene Integration Pattern in F4 hGH-Transgenic Common Carp(Cyprinus carpio L ) 87Integration of Double-Fluorescence Expression Vectors into Zebrafish Genome for theSelection of Site-Directed Knockout/Knockin 101Site-Directed Gene Integration in Transgenic Zebrafish Mediated by Cre RecombinaseUsing A Combination of Mutant Lox Sites 114Inhibition of No Tail (ntl) Gene Expression in Zebrafish by External Guide Sequence(EGS) Technique 128Non-Homologous End Joining Plays A Key Role in Transgene Concatemer Formation inTransgenic Zebrafish Embryos 137Integration Mechanisms of Transgenes and Population Fitness of GH Transgenic Fish 157Gene Transfer and Mutagenesis Mediated by Sleeping Beauty Transposon in Nile Tilapia(Oreochromis niloticus) 172Targeted Expression in Zebrafish Primordial Germ Cells by Cre/LoxP and Gal4/UASSystems 189Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes inZebrafish Using TALENs 211Efficient Ligase 3-Dependent Microhomology-Mediated End Joining Repair of DNADouble-Strand Breaks in Zebrafish Embryos 225µÚÈý²¿·Ö ÍâÔ´»ùÒò±í´ïµÄÉúÀí¹¦ÄÜHormonal Replacement Therapy in Fish: Human Growth Hormone Gene Function inHypophysectomized Carp 249Food Consumption and Energy Budget in MThGH-Transgenic F2 Red Carp (Cyprinuscarpio L red var ) 263Whole-Body Amino Acid Pattern of F4 Human Growth Hormone Gene-Transgenic RedCommon Carp (Cyprinus carpio) Fed Diets with Different Protein Levels 269Transgene for Growth Hormone in Common Carp (Cyprinus carpio L ) Promotes ThymusDevelopment 276Effects of the ¡°All-Fish¡± Growth Hormone Transgene Expression on Non-SpecificImmune Functions of Common Carp£¬ Cyprinus carpio L 287Rapid Growth Cost in ¡°All-Fish¡± Growth Hormone Gene Transgenic Carp: ReducedCritical Swimming Speed 298Growth and Energy Budget of F2 ¡®All-Fish¡¯ Growth Hormone Gene Transgenic CommonCarp 307Fast-Growing Transgenic Common Carp Mounting Compensatory Growth 321Metabolism Traits of ¡®All-Fish¡¯ Growth Hormone Transgenic Common Carp (Cyprinuscarpio L ) 334Enzyme-Linked Immunosorbent Assay of Changes in Serum Levels of GrowthHormone (cGH) in Common Carps (Cyprinus carpio) 350The Hematological Response to Exhaustive Exercise in ¡®All-Fish¡¯ GrowthHormone Transgenic Common Carp (Cyprinus carpio L ) 362Acute and Chronic Un-Ionized Ammonia Toxicity to ¡®All-Fish¡¯ Growth HormoneTransgenic Common Carp (Cyprinus carpio L ) 376Effects of Growth Hormone (GH) Transgene and Nutrition on Growth and BoneDevelopment in Common Carp 386Increased Food Intake in Growth Hormone-Transgenic Common Carp (Cyprinus carpio L )May Be Mediated by Upregulating Agouti-Related Protein (AgRP) 401µÚËIJ¿·Ö ת»ùÒò»ÆºÓÀðµÄÉú̬°²È«Ñо¿Progress in the Evaluation of Transgenic Fish for Possible Ecological Risk and ItsContainment Strategies 423Reduced Swimming Abilities in Fast-Growing Transgenic Common Carp Cyprinus carpioAssociated with Their Morphological Variations 433Elevated Ability to Compete for Limited Food Resources by ¡®All-Fish¡¯ GrowthHormone Transgenic Common Carp Cyprinus carpio 446Increased Mortality of Growth-Enhanced Transgenic Common Carp (Cyprinus carpio L )Under Short-Term Predation Risk 462Behavioral Alterations in GH Transgenic Common Carp May Explain EnhancedCompetitive Feeding Ability 471Risk-Taking Behaviour May Explain High Predation Mortality of GH-TransgenicCommon Carp Cyprinus carpio 489Transgenic Common Carp Do Not Have the Ability to Expand Populations 506Rapid Growth Increases Intrinsic Predation Risk in Genetically Modified Cyprinus carpio:Implications for Environmental Risk 518µÚÎ岿·Ö ÓãÀàÉúÖ³µ÷¿ØÓëת»ùÒòÓãµÄÓýÐÔ¿ØÖÆÑо¿A Perspective on Fish Gonad Manipulation for Biotechnical Applications 535Cloning and Expression Analysis in Mature Individuals of Two Chicken Type-II GnRH(cGnRH-II) Genes in Common Carp (Cyprinus carpio) 546Identification and Characterization of A Novel Splice Variant of Gonadotropin ¦ÁSubunit in the Common Carp Cyprinus carpio 560Antisense for Gonadotropin-Releasing Hormone Reduces Gonadotropin