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Preface Part I Gene Cloning Chapter 1 Introduction to Gene Cloning 1£®1 Brief Overview 1£®2 Historical Events 1£®3 Significance and Alertness Chapter 2 Gene and Genome 2£®1 Discovery of Hereditary Factor 2£®2 Basic Knowledge of Gene 2£®3 Sequence Signals Involved with Gene Expression 2£®4 About Genomic and Metagenomic Studies Chapter 3 Basic Techniques for Experiments with DNA 3£®1 Enzymatic Tools for Gene Manipulation 3£®2 Oligo-nucleotide Tools 3£®3 DNA Purification 3£®4 Electrophoresis 3£®5 In Vitro Synthesis of DNA 3£®6 DNA Sequencing 3£®7 Macromolecular Hybridization and Blotting 3£®8 PGR Techniques Chapter 4 Cloning Procedure 4£®1 Basic Procedure of Cloning 4£®2 Selection of Gloning Vectors 4£®3 Preparation of Donor DNA 4£®4 Joining of Donor and Vector by Ligation 4£®5 Selection of Host Gells * 4£®6 Introduction of Recombinant DNA into Host Gells 4£®7 Selection£¬ Screening and Sub-cloning \" 4£®8 Derivative Technology Based on Cloning Chapter 5 Application andlmpacts 5£®1 Initiation of Recombinant DNA Industry 5£®2 GeneraIAspects of Application 5£®3 Gontribution and Impact Part II Cell Engineering Chapter 6 Introduction on Cell Engineering 6£®1 Brief Overview 6£®2 Essentials of CeII Engineering 6£®3 Progress Glues Chapter 7 Cell Fusion and Hybridization 7£®1 General Aspects 7£®2 Protoplast Technique 7£®3 Basic Methods of Cell Fusion 7£®4 Fusant Screening 7£®5 Application of Cell Fusion Chapter 8 Plant Tissue and Cell Culture 8£®1 Plant Tissue Culture System 8£®2 Gene Transfer to Plants 8£®3 Artificial Seeds and Virus-free Plants Chapter 9 Animal Tissue and Cell Culture 9£®1 Establishment of Animal Tissue Culture System 9£®2 Gene Transfer to AnimaICell 9£®3 AnimaIHybrids and Hybridoma 9£®4 Approaches of Stem Cell Research Chapter 10 Application Examples from Cell Engineering 10£®1 Bioproducts from Gell Engineering 10£®2 Breeding of New Agronomic Varieties 10£®3 Transgenic Animals as Models for Medical Research Part III Enzyme Technology Chapter 11 Fundamental Nature of Enzyme 11£®1 Definition£¬ Basic Nature and Glassification of Enzyme 11£®2 Determination of Enzyme Activities 11£®3 Factors Affecting Enzyme Activity 11£®4 Resources of Enzyme 11£®5 Uses of Enzyme Chapter 12 Enzyme Biosynthesis and Products 12£®1 Bio-resources of Enzyme 12£®2 About Enzyme Synthesis ¡­¡­ Part IV Industrial Fermentation Appendix